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KMID : 0379520020180030293
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한국독성학회지
2002 Volume.18 No. 3 p.293 ~ p.300
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Effects of Chemical Carcinogens on the Aldehyde Metabolic Enzymes and Antioxidant Enzymes in Clone 9 Cell
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Kim Hyun-Bae
Yum Young-Na
Lee Mi-Young
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Abstract
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Chemical carcinogen-induced alteration of aldehyde metabolic enzymes were examined in clone 9 cell. Diethylnitrosamine (DENA), N-nitrosoethylurea (NEU) and N-nitrosomorpholine (NNM) were wed as model carcinogens. Changes in enzyme activities by repetitive treatment of DENA, NEU or NNM were analyzed in terms of specific activities and activity stainings of the enzymes on the gel. Upon treatment of DENA, lipid peroxide level increased upto 10 fold, indicating strong oxidative stress state of the cell. Notable enhancement of ADH and ALDH activity occurred after DENA treatment, while glutathione-S-transferase activity was slightly increased. Furthermore, about 2.5 fold higher superoxide dismutase (SOD) activity was detected during deactivation of catalase (CAT) activity by repetitive treatment of DENA. However in NEU-treated cell, about 2.3 fold higher ALDH activity was found while ADH activity was slightly increased. Notable increase CAT and SOD could also be found. In contrast, maximum 3.5 fold higher CAT activity occurred during SOD deactivation in NNM-treated cell. These results suggest that there might be different enzymatic responses in relation to cell protection against DENA, NEU or NNM
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KEYWORD
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Diethylnitrosamine, N-nitrosoethylurea, N-nitrosomorpholine, Lipid peroxidation, Aldehyde metabolic enzymes, Antioxidant enzymes, Clone 9 cell
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